Molecular Cloning and Sequence Analysis of a gene encoding WD Repeat-Containing Protein 13 from Ginkgo biloba
نویسندگان
چکیده
The WD-repeat proteins are found in eukaryotes and play an important role in the regulation of a wide variety of cellular functions including signal transduction, pre-mRNA processing and cytoskeleton assembly. In this study, a cDNA encoding a WD repeat-containing protein 13 (GbWDR13) was cloned by PCR from Ginkgo biloba. The length of GbWDR13 gene is 1500 bp, encoding 496 amino acids. The predicted amino acid sequence of GbWDR13 containing a WD40 domain display extensive homology to WD-repeat amino acids from other plants including Vitis vinifera, Solanum lycopersicum, Phoenix dactylifera and Cicer arietinum. Phylogenetic analysis showed that the GbWDR13 has a closer relationship with WDR13 from Gymnosperms than from other plants, suggesting it may be from the same ancestor as WD-repeat proteins from other gymnosperms. The isolation and sequence analysis of GbWDR13 gene provided a foundation for further studying the function of WD-repeat proteins in
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